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High Five細胞

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High Five(正式名稱為BTI-Tn-5B1-4)是一種昆蟲細胞系,起源於粉紋夜蛾英语Cabbage looper卵巢細胞[1]。 它是由美國的植物研究所BTI英语Boyce Thompson Institute發現的。High Five 細胞是使用桿狀病毒轉染,進行重組蛋白表達的其中一個最常用細胞系,並且比Sf9細胞等鱗翅類昆蟲細胞系表達更多的重組蛋白[2][3][4]。它們可以在沒有血清的情況下生長,並且可以以鬆散的附著狀態或懸濁液培養[5] 。High Five細胞可產生大量的小分子核糖核酸小分子干擾核糖核酸piRNA,使其適合於研究以上三種類型的小沉默核糖核酸[6]

參考資料

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  1. ^ Wickham, TJ; Davis, T; Granados, RR; Shuler, ML; Wood, HA. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.. Biotechnology progress. NaN, 8 (5): 391–6 [2019-11-23]. PMID 1369220. doi:10.1021/bp00017a003. 
  2. ^ Davis, TR; Trotter, KM; Granados, RR; Wood, HA. Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation and secretion.. Bio/technology (Nature Publishing Company). 1992-10, 10 (10): 1148–50 [2019-11-23]. PMID 1368794. doi:10.1038/nbt1092-1148. 
  3. ^ Wickham, TJ; Nemerow, GR. Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system.. Biotechnology progress. NaN, 9 (1): 25–30 [2019-11-23]. PMID 7764044. doi:10.1021/bp00019a004. 
  4. ^ Granados RR, Guoxun L, Derksen AC, McKenna KA. A New Insect Cell Line from Trichoplusia ni (BTI-Tn-5B1-4) Susceptible to Trichoplusia ni Single Enveloped Nuclear Polyhedrosis. Virus. J. Invertebr. Pathol. 1994, 64: 260–266. doi:10.1016/s0022-2011(94)90400-6. 
  5. ^ Invitrogen. Cell Lines. Growth and Maintenance of Insect cell lines (PDF). A.0. Thermo Fisher Scientific. 31 March 2015 [2015-11-09]. (原始内容存档 (PDF)于2016-03-04).  |number=被忽略 (帮助)
  6. ^ Fu, Yu; Yang, Yujing; Zhang, Han; Farley, Gwen; Wang, Junling; Quarles, Kaycee A; Weng, Zhiping; Zamore, Phillip D. The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology. eLife. 2018-01-29, 7 [2019-11-23]. ISSN 2050-084X. PMC 5844692可免费查阅. doi:10.7554/eLife.31628. (原始内容存档于2019-11-23) (英语). 

外部鏈接

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