KG-1

KG-1是一种假二倍体人类急性骨髓性白血病细胞系，最初分离自一名59岁患有红白血病（erythroleukemia）而后来又发展成急性骨髓性白血病的白人男性患者的骨髓。KG-1细胞在8号染色体的长臂上具有部分“hexasomy”，这或能够解释KG-1细胞中AML1-ETO融合基因的形成速率明显更高的原因^[1]。KG-1细胞在形态上类似急性骨髓性白血病细胞，表现为明显的多形化，骨髓母细胞（英语：Myeloblast）和骨髓细胞占优势，同时又有少量的成熟粒细胞，以及微量的巨噬细胞和嗜酸性球。

研究用途

KG-1细胞及分化程度较低的变异亚系KG-1a细胞目前常用于科学研究上。KG-1细胞可以研究与白血病相关的FGFR1（英语：Fibroblast growth factor receptor 1）融合物，以及是分析针对FGFR1融合物的小分子抑制剂的体外模型^[2]。此外，KG-1细胞被认为是人类树突状细胞祖细胞的模型。目前已经知道介白素-18可以诱导这些细胞向树突状表型生长，并且在该模型中能够介导γ-干扰素的产生^[3]。KG-1细胞的变异亚系KG-1a细胞则失去了髓样特征，获得新的核型标记，并且具有三个与未成熟T细胞相关的特征：从种系基因转录的T细胞受体 β mRNA的低水平表达、T3 δ mRNA和细胞内T3蛋白的高水平表达，以及CD7/gp40 T细胞相关膜抗原的表达^[4]。目前已知KG-1a细胞可以用于研究细胞对集落刺激因子的反应，以及研究白血病细胞中的抗原表达^[5]。

参考资料

^ Mrózek, K; Tanner, SM; Heinonen, K; Bloomfield, CD. Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization.. Genes, chromosomes & cancer. 2003-11, 38 (3): 249–52 [2020-02-19]. PMID 14506699. doi:10.1002/gcc.10274. ^{[永久失效链接]}
^ Gu, TL; Goss, VL; Reeves, C; Popova, L; Nardone, J; Macneill, J; Walters, DK; Wang, Y; Rush, J; Comb, MJ; Druker, BJ; Polakiewicz, RD. Phosphotyrosine profiling identifies the KG-1 cell line as a model for the study of FGFR1 fusions in acute myeloid leukemia.. Blood. 2006-12-15, 108 (13): 4202–4 [2020-02-19]. PMID 16946300. doi:10.1182/blood-2006-06-026666. ^{[永久失效链接]}
^ Bachmann, M; Dragoi, C; Poleganov, MA; Pfeilschifter, J; Mühl, H. Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells.. Molecular cancer therapeutics. 2007-02, 6 (2): 723–31 [2020-02-19]. PMID 17308068. doi:10.1158/1535-7163.MCT-06-0505. ^{[永久失效链接]}
^ Furley, AJ; Reeves, BR; Mizutani, S; Altass, LJ; Watt, SM; Jacob, MC; van den Elsen, P; Terhorst, C; Greaves, MF. Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines.. Blood. 1986-11, 68 (5): 1101–7 [2020-02-19]. PMID 3094604. ^{[永久失效链接]}
^ Koeffler, HP; Billing, R; Lusis, AJ; Sparkes, R; Golde, DW. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1).. Blood. 1980-08, 56 (2): 265–73 [2020-02-19]. PMID 6967340. ^{[永久失效链接]}

外部链接

Cellosaurus entry for KG-1 （页面存档备份，存于互联网档案馆）

[1] Mrózek, K; Tanner, SM; Heinonen, K; Bloomfield, CD. Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization.. Genes, chromosomes & cancer. 2003-11, 38 (3): 249–52 [2020-02-19]. PMID 14506699. doi:10.1002/gcc.10274. ^{[永久失效链接]}

[2] Gu, TL; Goss, VL; Reeves, C; Popova, L; Nardone, J; Macneill, J; Walters, DK; Wang, Y; Rush, J; Comb, MJ; Druker, BJ; Polakiewicz, RD. Phosphotyrosine profiling identifies the KG-1 cell line as a model for the study of FGFR1 fusions in acute myeloid leukemia.. Blood. 2006-12-15, 108 (13): 4202–4 [2020-02-19]. PMID 16946300. doi:10.1182/blood-2006-06-026666. ^{[永久失效链接]}

[3] Bachmann, M; Dragoi, C; Poleganov, MA; Pfeilschifter, J; Mühl, H. Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells.. Molecular cancer therapeutics. 2007-02, 6 (2): 723–31 [2020-02-19]. PMID 17308068. doi:10.1158/1535-7163.MCT-06-0505. ^{[永久失效链接]}

[4] Furley, AJ; Reeves, BR; Mizutani, S; Altass, LJ; Watt, SM; Jacob, MC; van den Elsen, P; Terhorst, C; Greaves, MF. Divergent molecular phenotypes of KG1 and KG1a myeloid cell lines.. Blood. 1986-11, 68 (5): 1101–7 [2020-02-19]. PMID 3094604. ^{[永久失效链接]}

[5] Koeffler, HP; Billing, R; Lusis, AJ; Sparkes, R; Golde, DW. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1).. Blood. 1980-08, 56 (2): 265–73 [2020-02-19]. PMID 6967340. ^{[永久失效链接]}

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