小发夹RNA

小发夹RNA（英语：short hairpin RNA，缩写shRNA）是一种形成急转弯（hairpin turn）结构的RNA序列，可以经由RNA干扰（RNAi）使基因表现沉默化。shRNA可利用载体导入细胞当中，并借由U6启动子来确保shRNA的表现。另外，shRNA可经由切割转变成为siRNA

参考文献

McIntyre G, Fanning G. Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnol. 2006, 6: 1. PMID 16396676. ^{[永久失效链接]}
Harper S, Staber P, He X, Eliason S, Martins I, Mao Q, Yang L, Kotin R, Paulson H, Davidson B. RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model. Proc. Natl. Acad. Sci. U.S.A. 2005, 102 (16): 5820–5. PMID 15811941. ^{[永久失效链接]}
Nielsen M, Pedersen F, Kjems J. Molecular strategies to inhibit HIV-1 replication. Retrovirology. 2005, 2: 10. PMID 15715913. ^{[永久失效链接]}
Paddison P, Caudy A, Bernstein E, Hannon G, Conklin D. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 2002, 16 (8): 948–58 [2007-07-25]. PMID 11959843. （原始内容存档于2007-09-27）.
Cao W, Hunter R, Strnatka D, McQueen C, Erickson R. DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response (PDF). J. Appl. Genet. 2005, 46 (2): 217–25 [2007-07-25]. PMID 15876690. （原始内容存档 (PDF)于2020-11-27）.

参见