硼酸锂缓冲液
硼酸锂缓冲液（英语：lithium borate buffer）、LB缓冲液（英语：LB buffer），是用于琼脂糖电泳的缓冲溶液，常用于分离核酸，如DNA、RNA。由硼酸锂（氢氧化锂一水合物和硼酸）组成。
LB(R) is a registered (USPTO) trademark of Faster Better Media LLC, which owns US patent 7,163,610 covering low-conductance lithium borate polynucleotide electrophoresis.
Lithium Borate buffer has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-50 V/cm as compared to 5-10 V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time.[1] Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected with lithium boric acid gels. [2][3]
SB buffer containing sodium borate is similar to lithium borate and has nearly all of its advantages at a somewhat lower cost, but the lithium buffer permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions and has a better resolution for fragments above 4kb.
参见
[编辑]外部链接
[编辑]- 硼酸钠作为电泳缓冲液 （页面存档备份，存于互联网档案馆）
参考文献
[编辑]- ^ Brody, J.R., Kern, S.E. (2004): History and principles of conductive media for standard DNA electrophoresis. Anal Biochem. 333(1):1-13. doi:10.1016/j.ab.2004.05.054 PMID 15351274 PDF （页面存档备份，存于互联网档案馆）
- ^ Sodium boric acid: a tris-free, cooler conductive medium for DNA electrophoresis. Biotechniques 36(2):214-215. PMID 14989083 PDF （页面存档备份，存于互联网档案馆）
- ^ Brody, J.R., Calhoun, E.S., Gallmeier, E., Creavalle, T.D., Kern, S.E. (2004): Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. Biotechniques 37(4):598-602. PMID 15517972 PDF[永久失效链接]
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