ERG3

Sterol C-5 desaturase
标识
生物	Saccharomyces cerevisiae
符号	ERG3
可选符号	SC5D, SYR1
Entrez	850745
RefSeq （mRNA）	NM_001181943
其他数据
EC number	1.14.19.20
染色体	XII: 0.25 - 0.25 Mb

ERG3 或甾醇-C5-去饱和酶（英語：Sterol C5-desaturase）是真菌釀酒酵母（Saccharomyces cerevisiae）的涉及麦角固醇生物合成的一种酶，与人类基因组中的CS5D同源^[1]，可定位于内质网或囊泡中^[2]^[3]，催化表甾醇的C5(6)脱氢形成双键变为5-脱氢表甾醇，5-脱氢表甾醇之后经历ERG5、ERG4等多个酶的转化最终变为麦角固醇^[4]^[5]。

参考文献

^ Hemmi K, Julmanop C, Hirata D, Tsuchiya E, Takemoto JY, Miyakawa T. The physiological roles of membrane ergosterol as revealed by the phenotypes of syr1/erg3 null mutant of Saccharomyces cerevisiae. Bioscience, Biotechnology, and Biochemistry. March 1995, 59 (3): 482–6. PMID 7766188. doi:10.1271/BBB.59.482.
^ Natter K, Leitner P, Faschinger A, Wolinski H, McCraith S, Fields S, Kohlwein SD. The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy. Molecular & Cellular Proteomics. May 2005, 4 (5): 662–72. PMID 15716577. doi:10.1074/mcp.M400123-MCP200 .
^ Poklepovich TJ, Rinaldi MA, Tomazic ML, Favale NO, Turkewitz AP, Nudel CB, Nusblat AD. The cytochrome b5 dependent C-5(6) sterol desaturase DES5A from the endoplasmic reticulum of Tetrahymena thermophila complements ergosterol biosynthesis mutants in Saccharomyces cerevisiae. Steroids. November 2012, 77 (13): 1313–20. PMC 3501532 . PMID 22982564. doi:10.1016/j.steroids.2012.08.015.
^ Brumfield KM, Moroney JV, Moore TS, Simms TA, Donze D. Functional characterization of the Chlamydomonas reinhardtii ERG3 ortholog, a gene involved in the biosynthesis of ergosterol. PLOS ONE. January 2010, 5 (1): e8659. Bibcode:2010PLoSO...5.8659B. PMC 2799552 . PMID 20084111. doi:10.1371/journal.pone.0008659.
^ Miyazaki T, Miyazaki Y, Izumikawa K, Kakeya H, Miyakoshi S, Bennett JE, Kohno S. Fluconazole treatment is effective against a Candida albicans erg3/erg3 mutant in vivo despite in vitro resistance. Antimicrobial Agents and Chemotherapy. February 2006, 50 (2): 580–6. PMC 1366932 . PMID 16436713. doi:10.1128/AAC.50.2.580-586.2006.
^ Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier CP, Gregory BD, et al. The Arabidopsis dwf7/ste1 mutant is defective in the delta7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. The Plant Cell. February 1999, 11 (2): 207–21. PMC 144158 . PMID 9927639. doi:10.1105/tpc.11.2.207.

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[1] Hemmi K, Julmanop C, Hirata D, Tsuchiya E, Takemoto JY, Miyakawa T. The physiological roles of membrane ergosterol as revealed by the phenotypes of syr1/erg3 null mutant of Saccharomyces cerevisiae. Bioscience, Biotechnology, and Biochemistry. March 1995, 59 (3): 482–6. PMID 7766188. doi:10.1271/BBB.59.482.

[2] Natter K, Leitner P, Faschinger A, Wolinski H, McCraith S, Fields S, Kohlwein SD. The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy. Molecular & Cellular Proteomics. May 2005, 4 (5): 662–72. PMID 15716577. doi:10.1074/mcp.M400123-MCP200 .

[3] Poklepovich TJ, Rinaldi MA, Tomazic ML, Favale NO, Turkewitz AP, Nudel CB, Nusblat AD. The cytochrome b5 dependent C-5(6) sterol desaturase DES5A from the endoplasmic reticulum of Tetrahymena thermophila complements ergosterol biosynthesis mutants in Saccharomyces cerevisiae. Steroids. November 2012, 77 (13): 1313–20. PMC 3501532 . PMID 22982564. doi:10.1016/j.steroids.2012.08.015.

[4] Brumfield KM, Moroney JV, Moore TS, Simms TA, Donze D. Functional characterization of the Chlamydomonas reinhardtii ERG3 ortholog, a gene involved in the biosynthesis of ergosterol. PLOS ONE. January 2010, 5 (1): e8659. Bibcode:2010PLoSO...5.8659B. PMC 2799552 . PMID 20084111. doi:10.1371/journal.pone.0008659.

[5] Miyazaki T, Miyazaki Y, Izumikawa K, Kakeya H, Miyakoshi S, Bennett JE, Kohno S. Fluconazole treatment is effective against a Candida albicans erg3/erg3 mutant in vivo despite in vitro resistance. Antimicrobial Agents and Chemotherapy. February 2006, 50 (2): 580–6. PMC 1366932 . PMID 16436713. doi:10.1128/AAC.50.2.580-586.2006.

[6] Choe S, Noguchi T, Fujioka S, Takatsuto S, Tissier CP, Gregory BD, et al. The Arabidopsis dwf7/ste1 mutant is defective in the delta7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis. The Plant Cell. February 1999, 11 (2): 207–21. PMC 144158 . PMID 9927639. doi:10.1105/tpc.11.2.207.

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