BeWo

BeWo (亦稱ATCC CCL-98) 是人類胎盤絨毛膜癌細胞系^[1]，較常應用於胎盤屏障生理功能^[2]及人類免疫缺陷病毒的研究^[3]，而超三倍體 JEG-3細胞則是其衍生的克隆。BeWo細胞具有可產生大量層粘連蛋白的細胞外基質^[4]，並且BeWo細胞會表達雌激素 (雌酮、雌二醇及雌三醇)、孕激素、人絨毛膜促性腺激素、胎盤催乳素及角蛋白等物質。目前已知BeWo的b30亞克隆可在雙房室 (bicameral chambers) 的可滲透膜上生長，以形成融合性的細胞層，從而確定營養從頂端表面吸收的速率，以及從基底外側膜中流出的速率^[5]。

有研究指出沈默LOX在Bewo細胞中的表達，會明顯降低Bewo細胞侵襲及轉移能力。因為過度表達LOX可以改變腫瘤細胞外基質的結構，並且激活特定通路及上調MMPs類的表達，使乳癌的轉移侵襲速度因基底膜被降解而加速^[6]。

參考資料

^ Pattillo, RA; Gey, GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.. Cancer research. 1968-07, 28 (7): 1231–6 [2019-12-08]. PMID 4299001.
^ Zhou, F; Hong, M; You, G. Regulation of human organic anion transporter 4 by progesterone and protein kinase C in human placental BeWo cells.. American journal of physiology. Endocrinology and metabolism. 2007-07, 293 (1): E57–61 [2019-12-08]. PMID 17341544. doi:10.1152/ajpendo.00696.2006.
^ Ayouba, A; Cannou, C; Nugeyre, MT; Barré-Sinoussi, F; Menu, E. Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro.. Retrovirology. 2008-03-31, 5: 31 [2019-12-08]. PMID 18377645. doi:10.1186/1742-4690-5-31.
^ Church, HJ; Aplin, JD. BeWo choriocarcinoma cells produce laminin 10.. The Biochemical journal. 1998-06-01,. 332 ( Pt 2): 491–8 [2019-12-08]. PMID 9601079. doi:10.1042/bj3320491.
^ Heaton, SJ; Eady, JJ; Parker, ML; Gotts, KL; Dainty, JR; Fairweather-Tait, SJ; McArdle, HJ; Srai, KS; Elliott, RM. The use of BeWo cells as an in vitro model for placental iron transport.. American journal of physiology. Cell physiology. 2008-11, 295 (5): C1445–53 [2019-12-08]. PMID 18815225. doi:10.1152/ajpcell.00286.2008.
^ 王璟; 郭影; 趙向寨; 李萍. LOX基因對人絨癌Bewo細胞侵襲轉移的影響及意義. 中國老年學雜誌. 2014, (11): 3074–6. doi:10.3969/j.issn.1005-9202.2014.11.076.

外部連結

Cellosaurus entry for BeWo（页面存档备份，存于互联网档案馆）

[1] Pattillo, RA; Gey, GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.. Cancer research. 1968-07, 28 (7): 1231–6 [2019-12-08]. PMID 4299001.

[2] Zhou, F; Hong, M; You, G. Regulation of human organic anion transporter 4 by progesterone and protein kinase C in human placental BeWo cells.. American journal of physiology. Endocrinology and metabolism. 2007-07, 293 (1): E57–61 [2019-12-08]. PMID 17341544. doi:10.1152/ajpendo.00696.2006.

[3] Ayouba, A; Cannou, C; Nugeyre, MT; Barré-Sinoussi, F; Menu, E. Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro.. Retrovirology. 2008-03-31, 5: 31 [2019-12-08]. PMID 18377645. doi:10.1186/1742-4690-5-31.

[4] Church, HJ; Aplin, JD. BeWo choriocarcinoma cells produce laminin 10.. The Biochemical journal. 1998-06-01,. 332 ( Pt 2): 491–8 [2019-12-08]. PMID 9601079. doi:10.1042/bj3320491.

[5] Heaton, SJ; Eady, JJ; Parker, ML; Gotts, KL; Dainty, JR; Fairweather-Tait, SJ; McArdle, HJ; Srai, KS; Elliott, RM. The use of BeWo cells as an in vitro model for placental iron transport.. American journal of physiology. Cell physiology. 2008-11, 295 (5): C1445–53 [2019-12-08]. PMID 18815225. doi:10.1152/ajpcell.00286.2008.

[6] 王璟; 郭影; 趙向寨; 李萍. LOX基因對人絨癌Bewo細胞侵襲轉移的影響及意義. 中國老年學雜誌. 2014, (11): 3074–6. doi:10.3969/j.issn.1005-9202.2014.11.076.

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